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. 2009 Feb 3;37(6):1886–1896. doi: 10.1093/nar/gkp036

Figure 3.

Figure 3.

Dual-label competitive binding assay. (a) Scheme of the competitive binding experiments with two labeled RNAs. Complexes of the 5′ domain were separated on 8% polyacrylamide gels in TBE, while complexes of the 5WJ variants were separated on 6% polyacrylamide gels in TKM2, as described in ‘Material and Methods’ sections. (b) Distribution of S4 between 5′ domain and 5WJ RNAs. The counts in each complex and in free RNA were quantified and used to calculate Krel [Equation (2)]. For the 5WJ RNA, average Krel = 0.6 ± 0.1. The amount of labeled 5WJ complex decreases as unlabeled 5WJ RNA is added because its specific activity decreases. See Table 1 for further data.