Table 1.
Oligonucleotides used in this study
| Oligonucleotides | Sequence |
|---|---|
| Adaptor oligonucleotides | |
| Adaptor-Ashort | 5′CTGAGACAGGGAGGGAACAGATGGGACACGCAGGGATGAG |
| Adaptor-Along | 5′CCATCTCATCCCTGCGTGTCCCATCTGTTCCCTCCCTGTCTCAG |
| Adaptor-Bshort | 5′CTGAGACACGCAACAGGGGATAGGCAAGGCACACAGGGGA |
| Adaptor-Blong Biotin labelled | 5′(Bio)CCTATCCCCTGTGTGCCTTGCCTATCCCCTGTTGCGTGTCTCAG |
| Library amplification PCR primers | |
| Amplifier-Aout | 5′TCCCTGCGTGTCCCATCTGT |
| Amplifier-Bout | 5′CCCTGTGTGCCTTGCCTATCC |
| Amplifier-Ain | 5′CCATCTGTTCCCTCCCTGTC |
| Amplifier-Bin | 5′CCTATCCCCTGTTGCGTGTC |
| Analytical PCR primers | |
| Orf-2out | 5′CTAGCTTGACGAGTTTCTCAC |
| Orf-2in | 5′(Alexa488)GACGAGTTTCTCACTAAGCCA |
| Nuc-2out | 5′GGAGCATGAGATAATTAAATGGG |
| Nuc-2in | 5′(Alexa488)GGGCCAATGACTATGTGGTAACAC |
| Nuc-1out | 5′CTGTCATTGTTTGGTGGATGT |
| Nuc-1in | 5′(Alexa488)CATCGTGGATGGCTTCGATG |
| Nuc+2out | 5′CCGTCTGGCTTGCCGTTGACCC |
| Nuc+2in | 5′(Alexa488)TTGCCGTTGACCCGGGCTGCAGTA |
| P442out | 5′GGGGGGTTGCAGGGATGGG |
| P442in | 5′(FAM)GGATGGGATCGAGCTCCAG |
| P689out | 5′GGTCGAGGATGCAGGGAAG |
| P689in | 5′(FAM)AAGAAGCGAAGAAGGTGCA |
| P900out | 5′ATAGGACAGGGAAGGGGATG |
| P900in | 5′(FAM)AGAGCGACGAGAGGAGTGA |
| Alfrout | 5′GATATTTGCATATGATACAGGCCCGCATTG |
| Aflrin | 5′(FAM)GGCCCGCATTGCGGATTGAACCG |
| areAclaIF | 5′ATCGATGGATCTATTAAGG |
| areAscaIR | 5′AGTACTAGCAGCTGGTTGCATTAC |