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. 2009 Feb 3;37(6):1897–1906. doi: 10.1093/nar/gkp049

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — RNAi-mediated downregulation of TbMP99 and/or TbMP100. (A) RT-PCR analysis of TbMP99 and TbMP100 single knockdown (99 skd and 100 skd) and TbMP99/TbMP100 double knockdown cells (99/100 dkd). Upon induction (6 days) with tetracycline (+tet) the mRNA level of either TbMP99, TbMP100 or of both transcripts was ≤5%. tub, α-tubulin. (B) Growth behavior of 99 skd, 100 skd and 99/100 dkd trypanosomes. (C) Autoadenylation of the editing ligases TbMP52 and TbMP48 in gradient fractionated mitochondrial extracts from wild-type (WT), 99 skd, 100 skd and 99/100 dkd trypanosomes prepared 6 days postinduction. Sub-20S and 20S fractions are indicated.