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Stable retention of 25-4 vector in vertebrate cell lines. The vertebrate cell lines carrying 25-4 were established by MMCT. Transferred 25-4 was confirmed by FISH using metaphase cells: CHO, mouse ES (TT2F), pig (PK15), chicken B cells (DT40), Xenopus (A6) and zebrafish (AB.9). Green signals show alphoid DNA and red signals show BAC vector. Arrowheads show 25-4 vector.
