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Gene expression from the 25-4 vector. (a) Gene insertion into the 25-4 vector. The EGFP gene was inserted into 25-4 vector using Cre-lox recombination in HT1080 cells. Successful recombinants were selected by puromycin resistance (puro). (b) EGFP expression levels in 25-4. Fluorescence of CMV-, SV-, EF1- or cyclin A-driven expression of EGFP in HT1080 cells.
