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Map of psbA minicircle in H. triquetra. The full psbA gene sequence is around 1 kb, while the rest is occupied by the NCR. The 9G-9A-9G region of around 450 bp within the NCR had similar amounts of different minicircles of one species. The full-length 1.1-kb NCR and partial 0.7-kb psbA gene, amplified with corresponding primer pairs, were used as probes for Southern blotting.
