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. 2009 Mar 6;37(6):e47. doi: 10.1093/nar/gkp099

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© 2009 The Author(s)

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — Characterization of an unknown small RNA in yeast tRNA preparation. (a) Isolation of an unknown small RNA by anion-exchange chromatography. A mixture of yeast tRNA (tRNA typeX, Sigma R9001; 100 μg) was applied to a TSKgel DNA-NPR column (4.6 × 75 mm; Toso) and eluted with an 80-min gradient of NH₄Cl (0.1–1.0 M) in 25 mM Tris–HCl buffer (pH 9.0) at a flow rate of 0.5 ml/min at 60°C. The fraction indicated by a double-headed arrow was collected, purified by reversed-phase chromatography (data not shown) and subjected to the LC–MS analysis after digestion with RNase T1 (see Materials and Methods section). (b) Base peak chromatogram of the RNase T1 digest of the small RNA.