Table 1.
Strain | Hydrogenase activitya | Urease activityb |
---|---|---|
Wild type | 1.4 ± 0.3 | 64 ± 23 |
HypA | <0.01 | 0.3 ± 0.1 |
HypB | <0.01 | 1.6 ± 0.5 |
HypC | <0.01 | 62 ± 19 |
HypD | <0.01 | 71 ± 11 |
HypE | <0.01 | 76 ± 17 |
HypF | <0.01 | 78 ± 14 |
hypA:kanHA | 1.2 ± 0.3 | 62 ± 8 |
nmol H2 oxid per min per 108 cells
µmole urea hydrolyzed per min per mg protein
Urease activity of hypA and hypB strains was fully restored by adding 5 µM nickel. Data is from Olson et al. 2001, Mehta et al. 2003a and Benoit et al. 2004