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. Author manuscript; available in PMC: 2009 Apr 4.
Published in final edited form as: Biometals. 2007 Jan 5;20(3-4):655–664. doi: 10.1007/s10534-006-9061-8

Table 1.

Ni-enzyme activities of Helicobacter pylori accessory protein mutants

Strain Hydrogenase activitya Urease activityb
Wild type 1.4 ± 0.3 64 ± 23
HypA <0.01 0.3 ± 0.1
HypB <0.01 1.6 ± 0.5
HypC <0.01 62 ± 19
HypD <0.01 71 ± 11
HypE <0.01 76 ± 17
HypF <0.01 78 ± 14
hypA:kanHA 1.2 ± 0.3 62 ± 8
a

nmol H2 oxid per min per 108 cells

b

µmole urea hydrolyzed per min per mg protein

Urease activity of hypA and hypB strains was fully restored by adding 5 µM nickel. Data is from Olson et al. 2001, Mehta et al. 2003a and Benoit et al. 2004