ApoC-III and apoA-V levels in lipoproteins. Aliquots of peak fractions (15 μl) in VLDL, HDL1, and HDL2 fractions were resolved on 4–20% SDS-polyacrylamide gels, followed by semiquantitative immunoblot assay using antibodies against apoC-III (A), apoA-V (B), and apoE (C). ApoA-V and apoE were undetectable in VLDL fractions using the same immunoblot assay. Protein bands were quantified by densitometry. Data are expressed as means ± SEM. * P < 0.05. Ctrl, control.