Fig. 7.

A: Plasma LCAT activity. Plasma was prepared from 16 h fasted mice after 2 weeks of hepatic apoA-V production and subjected to LCAT activity assay. The relative LCAT activity was compared between apoA-V and control (Ctrl) groups. B: Cholesterol efflux in macrophages. The ability of apoA-V-enriched HDL to conduct cholesterol efflux was assayed in cultured macrophages using 5 μl of plasma from apoA-V and control groups of fasted mice at the end of the 2 week study. C: Hepatic scavenger receptor class B type I (SR-BI) levels. D: Hepatic low density lipoprotein receptor (LDLR) levels. E: Hepatic HMG-CoA reductase levels. Aliquots of 20 mg of liver tissue were homogenized for either the preparation of total liver protein extracts or the enrichment of plasma membrane proteins, which were subjected to semiquantitative Western blot analysis. The relative levels of hepatic proteins SR-BI, HMG-CoA, and LDLR were compared between apoA-V and control groups using actin or the membrane-specific marker sodium potassium ATPase as an internal control. Data are expressed as means ± SEM. * P < 0.05.