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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: Biochem Biophys Res Commun. 2009 Feb 11;381(2):181–186. doi: 10.1016/j.bbrc.2009.02.020

Fig. 1.

Fig. 1

AM-251 and SR144528 inhibit 7-ketocholesterol induced apoptosis. Raw 264.7 macrophages were pretreated for 1 h with AM-251 or SR144528, prior to treatment with 7KC (10 μg/ml) as indicated. After 16 h, apoptosis was evaluated by (A) caspase-3 activity assay and (B) APOPercentage assay. (C) Caspase-3 activity induced in Raw 264.7 macrophages after a 6h treatment with staurosporine (1 μM) in the absence and presence of AM-251 or SR144528 as indicated. (D) Cell viability of Raw 264.7 macrophages incubated for 24 h in medium supplemented with AM-251 or SR144528, as indicated, was determined by a modified MTT assay and presented as the percentage ± SD of the mean of the control cells treated with vehicle only. * p<0.05