Table 2.
Scaled Fluorescence Signal Intensity Levels from Microarrays of Single Mouse Retinal Cells1
| Fluorescence signal intensities |
|||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| (a) | (b) | (c) | (d) | (e) | (f) | (g) | (h) | (i) | (j) | (k) | (l) | (m) | (n) |
| Probe set ID | Unigene | Gene name | P5 bipolar A3 | Cabp5 bipolar A3 | Cabp5 bipolar A4 | Cabp5 bipolar B1 | Cabp5 bipolar B2 | Adult rod 1 | Adult rod 2 | Adult Müller glia 1 | Adult Müller glia 15 | Ratio of signal intensities of bipolar cells to rod photoreceptor + Müller glial cells | P value |
| 1451826_at | Mm.103669 | Calcium binding protein 5 (Cabp5) | 7,874 | 21,964 | 18,135 | 40,043 | 37,690 | 395 | 23 | 155 | 73 | 155.6 | 0.0042 |
| 1424944_at | Mm.41456 | Purkinje cell protein 2 (Pcp2) | 13 | 7,342 | 4,364 | 259 | 4,527 | 41 | 21 | 224 | 12 | 44.3 | 0.0406 |
| 1450945_at | Mm.222178 | Protein kinase C α (Prkca) | 143 | 3,273 | 7,272 | 9,010 | 2,643 | 28 | 200 | 184 | 26 | 40.9 | 0.0243 |
| 1419628_at | Mm.4405 | C. elegans ceh-10 homeodomain-containing homolog (Chx10) | 4,718 | 24,762 | 22,820 | 53,259 | 73,768 | 168 | 24 | 12 | 18,707 | 7.6 | 0.0343 |
| 1438782_at | Mm.321683 | Contactin 4 (Cntn4) | 3 | 12,582 | 6,377 | 26,568 | 23,591 | 527 | 141 | 14 | 4 | 80.6 | 0.0240 |
| 1427482_a_at | Mm.119320 | Carbonic anhydrase 8 (Car8) | 96 | 6,773 | 18,800 | 24,431 | 25,708 | 620 | 48 | 244 | 50 | 63.0 | 0.0174 |
| 1453008_at | Mm.151594 | RIKEN cDNA 2300002D11 gene (2300002D11Rik) | 14,214 | 25,518 | 23,537 | 16,469 | 13,327 | 1,082 | 60 | 386 | 54 | 47.0 | 0.0002 |
| 1457946_at | Mm.134360 | Og9 homeobox gene (Og9x) | 191 | 6,663 | 9,945 | 17,860 | 18334 | 168 | 634 | 190 | 40 | 41.0 | 0.0165 |
| 1424547_at | Mm.342160 | Carbonic anhydrase 10 (Car10) | 106 | 915 | 1,109 | 3,104 | 658 | 111 | 33 | 24 | 66 | 20.2 | 0.0471 |
| 1436205_at | Mm.380307 | Neurofascin (Nfasc) | 221 | 8,476 | 9,099 | 2,554 | 9,313 | 858 | 302 | 211 | 12 | 17.2 | 0.0180 |
| 1425041_at | Mm.15655 | LIM homeobox protein 3 (Lhx3) | 10,281 | 56 | 42 | 71 | 7 | 411 | 93 | 386 | 116 | 8.3 | 0.2271 |
| 1419740_at | Mm.1372 | Phosphodiesterase 6B, cGMP, rod receptor, β polypeptide (Pde6b) | 21 | 7,981 | 20 | 38 | 193 | 514,284 | 167,133 | 28 | 22 | 0.010 | 0.0786 |
| 1451763_at | Mm.23793 | Cyclic nucleotide gated channel α1 (Cnga1) | 22 | 1,979 | 7 | 14 | 15 | 194,172 | 159,622 | 28 | 2,985 | 0.005 | 0.0443 |
| 1451617_at | Mm.2965 | Rhodopsin (Rho) | 5 | 955 | 9 | 26 | 3,523 | 710,723 | 272,295 | 12,406 | 16 | 0.004 | 0.0667 |
| 1418310_a_at | Mm.41653 | Retinaldehyde binding protein 1 (Rlbp1) | 3 | 4,521 | 26 | 28 | 13,236 | 34 | 7 | 168,251 | 109,338 | 0.051 | 0.0586 |
| 1426236_a_at | Mm.210745 | Glutamate-ammonia ligase (Glul) | 41 | 3,798 | 4 | 13 | 282 | 434 | 296 | 221,720 | 89,714 | 0.011 | 0.0684 |
Signals are shown from a P5 bipolar cell (column d), four bipolar cells transfected with a Cabp5:GFP reporter construct at P0 and picked from retinas after 8 days in culture (columns e–h), two adult rod photoreceptor cells (columns i–j), and two adult Müller glial cells (columns k,l). For each gene, the ratio of the average signal of bipolar cells to the average signal of all other cells is shown (column m). The average bipolar signal was compared with the average signal of all other cells by one-tailed Student's t-test, and resulting P values are shown (column n). Candidate and known bipolar cell-enriched genes had ratios greater than 7.6, whereas known rod photoreceptor and Müller glial cell-enriched genes had ratios less than 0.051.