Table 3.
Quantitation of Dissociated P14 Mouse Retinal Cells Double-Labeled with Fluorescent Riboprobes for Novel and Known Bipolar Cell-Enriched Markers1
| (a) |
(b) |
(c) |
(d) |
(e) |
(f) |
(g) |
(h) |
(i) |
(j) |
(k) |
|---|---|---|---|---|---|---|---|---|---|---|
| Experimental probe | Experimental | Pcp2 | Grm6 | Chx10 | Doubly labeled with experimental and Pcp2/Pcp2 | Doubly labeled with experimental and Grm6/Grm6 | Doubly labeled with experimental and Chx10/Chx10 | Doubly labeled with experimental and Pcp2/experimental | Doubly labeled with experimental and Grm6/experimental | Doubly labeled with experimental and Chx10/experimental |
| Og9x | 5.6 ± 0.5 | 9.5 ± 2.1 | 9.9 ± 2.1 | 13.9 ± 1.6 | 54.4 ± 4.0 | 33.5 ± 4.8 | 34.3 ± 6.1 | 69.8 ± 6.2 | 83.7 ± 2.3 | 98.8 ± 0.6 |
| Scgn | 5.2 ± 0.7 | 9.5 ± 1.7 | 10.0 ± 1.9 | 13.7 ± 4.7 | 10.5 ± 3.1 | 19.4 ± 4.5 | 17.1 ± 5.9 | 13.4 ± 1.6 | 34.4 ± 5.7 | 67.1 ± 20.3 |
| Cntn4 | 8.8 ± 2.4 | 6.4 ± 0.0 | 10.2 ± 5.2 | 14.0 ± 5.3 | 56.1 ± 14.5 | 33.3 ± 3.6 | 47.1 ± 7.2 | 44.7 ± 9.0 | 46.5 ± 13.9 | 59.2 ± 21.0 |
| Car8 | 7.0 ± 2.7 | 9.5 ± 2.1 | 9.9 ± 3.1 | 14.0 ± 2.7 | 62.8 ± 20.9 | 29.4 ± 14.8 | 47.6 ± 10.4 | 70.3 ± 5.8 | 75.1 ± 19.6 | 97.2 ± 2.8 |
| 2300002D11Rik | 7.7 ± 1.5 | 9.5 ± 2.3 | 9.9 ± 3.6 | 14.1 ± 3.7 | 42.6 ± 10.9 | 31.7 ± 6.4 | 48.3 ± 11.8 | 60.0 ± 9.0 | 71.7 ± 16.7 | 60.9 ± 18.8 |
| Nfasc | 8.0 ± 2.2 | 9.5 ± 2.3 | 10.5 ± 3.7 | 14.0 ± 4.4 | 29.4 ± 1.3 | 41.8 ± 1.7 | 33.9 ± 14.6 | 67.1 ± 15.5 | 33.6 ± 8.8 | 82.2 ± 9.5 |
| Trpm1 | 11.6 ± 3.1 | 9.5 ± 2.0 | 10.0 ± 1.3 | 14.1 ± 1.1 | 25.0 ± 5.2 | 76.3 ± 10.1 | 37.2 ± 5.8 | 37.7 ± 4.4 | 38.8 ± 8.6 | 83.3 ± 2.8 |
| 6330514A18Rik | 18.5 ± 5.7 | 7.5 ± 2.3 | 10.0 ± 3.0 | 13.5 ± 1.5 | 41.2 ± 18.8 | 58.7 ± 19.8 | 24.9 ± 8.1 | 17.8 ± 6.9 | 38.5 ± 20.8 | 46.5 ± 12.5 |
Percentages are shown as average ± SEM. Results from three retinas were independently quantitated for each experiment. Percentages of cells labeled with various experimental probes, a probe for the rod bipolar cell-enriched gene Pcp2, a probe for the ON bipolar cell gene Grm6, and a probe for the pan-bipolar cell gene Chx10, are shown. Columns b–e list the percentage of cells labeled with probes out of the total number of cells. Columns f–h list the percentages of cells doubly labeled with a probe for an experimental and a known gene out of the number of cells singly labeled with a probe for a known gene. These percentages are the fraction of cells positive for a known gene that are also positive for an experimental gene. Columns i–k list the percentages of cells doubly labeled with a probe for an experimental and a known gene out of the number of cells singly labeled with a probe for an experimental gene. These percentages are the fraction of cells positive for an experimental gene that are also positive for a known gene.