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. Author manuscript; available in PMC: 2009 Apr 5.
Published in final edited form as: J Immunol. 2006 Dec 1;177(11):8086–8094. doi: 10.4049/jimmunol.177.11.8086

FIGURE 1.

FIGURE 1

CD45 allele status of transplanted mice at day 60. Whole blood from transplanted mice was dual labeled with PE-anti-mCD45.1 and FITC-anti-mCD45.2 and analyzed by flow cytometry. The percentages of cells expressing the CD45.1 allele in mice were as follows: WT→WT, 98 ± 0.4%; KO→KO, 0.2 ± 0.1%; WT→KO, 96 ± 0.7%; and KO→WT, 5 ± 0.6%. The percentages of cells expressing the CD45.2 allele in each chimeric mice group were as follows: WT→WT, 0.3 ± 0.1%; KO→KO, 99 ± 0.2%; WT→KO, 2.5 ± 0.3%; and KO→WT, 95 ± 0.6%. There were 10–13 animals analyzed in each group, and this figure shows representative images from each transplanted group. Data from animals showing adequate reconstitution with donor marrow cells were included in subsequent analyses.