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. Author manuscript; available in PMC: 2009 Apr 5.
Published in final edited form as: J Immunol. 2006 Dec 1;177(11):8086–8094. doi: 10.4049/jimmunol.177.11.8086

FIGURE 2.

FIGURE 2

Duffy Ag expression in the lungs following total irradiation. A, Negative control immunostaining with nonspecific IgG. B, Duffy immunostaining with sheep anti-murine Duffy Ab. Immunoreactivity is present in endothelium in WT-irradiated mouse lung (indicated by red staining). C, Relative dfy gene expression in the lung following intratracheal LPS instillation. D, Relative dfy gene expression in the spleen following intratracheal LPS instillation. Gene expression was measured using quantitative real-time PCR in lung and spleen homogenates obtained from the four chimeric groups (WT→WT, KO→KO, WT→KO, and KO→KO). Gene expression was quantified using the ΔΔ CT method with 18S rRNA as the endogenous control. Unstimulated age-matched C57BL/6 mouse lung or spleen was used as the calibrator. Data represent three mice at each time for the four groups.