Figure 3.

Knocking down Rdl does not enhance learning in learning mutants defective in the cAMP pathway. A, Flies were trained using 3 pulses of electric shock during a CS+ odor presentation of 1 min. Flies carrying either Rdl RNAi2–7E2 or RNAi4–5E driven by c772-Gal4 showed significantly enhanced aversive learning. When combined with rut¹, they failed to show any enhancement. B, Flies were starved for 16 h and trained using 1 m sucrose during a CS+ odor presentation of 1.5 min. While flies carrying the two Rdl RNAi lines driven by the c772-Gal4 driver showed significantly enhanced appetitive learning, they failed to show any learning enhancement when combined with rut¹. C, Flies were trained using 3 or 12 pulses of electric shock during a CS+ odor presentation of 1 min. While flies carrying the c772-Gal4 driver and the Rdli8–10J transgene showed enhanced aversive learning on the wild-type background, they failed to show any learning enhancement on the rut²⁰⁸⁰ mutant background. D, Flies were trained using 3 pulses of electric shock during a CS+ odor presentation of 1 min. While flies carrying another MB driver c739-Gal4 and the Rdli2–7E2 transgene showed enhanced aversive learning on the wild-type background, they failed to show any learning enhancement on the NF1^P1 mutant background. For all panels, n = 6 for each group under each condition. Means ± SEM; *p < 0.05; **p < 0.01 (Fisher's PLSD); n.s., not significant.