. Author manuscript; available in PMC: 2009 Oct 7.

Published in final edited form as: Biochemistry. 2008 Sep 17;47(40):10587–10599. doi: 10.1021/bi8007033

Table 2.

Ca²⁺-dissociation Rates from CaM analyzed by Quin-2 and Stopped-flow Fluorimetry

	Rate 1	# Ca	Rate 2	# Ca	Total # Ca
CaM^a	9.09 ± 0.60	2	TFTM		2
CaM + CaMKII^b	9.86 ± 0.81	2.70 ± 0.33	0.82 ± 0.38	0.57 ± 0.21	3.27 ± 0.26
CaM + CaMKII^b + ADP	6.87 ± 1.26	1.55 ± 0.16	0.43 ± 0.05	2.62 ± 0.24	4.17 ± 0.30

Rates and amplitudes were fit with a one^a - or two^b-rate binding model. Amplitudes reported are the mol of Ca²⁺ recovered assuming 2 mol of Ca²⁺ were released from CaM in the absence of target. TFTM (too fast to measure) means that fraction of the reaction fell below the temporal resolution of our instrument (1.8 ms) and could not be reliably detected.