Table 5.
Quin-2 induced rates of Ca2+ dissociation from CaM constructs
| Construct | Rate 1 (s−1) | Amp. 1 (mol of Ca2+) | Rate 2 (s−1) | Amp. 2 (mol of Ca2+) |
|---|---|---|---|---|
| wtCaM | TFTM | 10 | 1.8 | |
| wtCaM + pep | 9.97 | 1.8 | 0.19 | 2.2 |
| CaMNN | 226 | 0.7 | 8.8 | 0.5 |
| CaMNN + pep | 6.9 | 3.7 | 0.35 | 0.3 |
| CaMCC | 16.15 | 3.2 | 3.6 | 0.7 |
| CaMCC + pep | 1.3 | 1.0 | 0.32 | 3.0 |
| CaM76–148 | 13.4 | 2.1 | ||
| CaM76–148 + pep | 0.56 | 2 | ||
| CaM81–148 | 43.3 | 1.9 | ||
| CaM81–148 + pep | 1.5 | 2 | ||
| CaM1–75 | TFTM | |||
| CaM1–75 + pep | 10.5 | 2 | ||
| CaM1–80 | TFTM | |||
| CaM1–80 + pep | 11.4 | 2 | ||
Rates and amplitudes were fit with a one- or two-rate binding model as necessary. Amplitudes reported are the mol of Ca2+ released per mol of protein assuming 100% of the amplitude was recovered in the presence of peptide. TFTM (too fast to measure) means that fraction of the reaction fell below the temporal resolution of our instrument (1.8 ms) and could not be reliably detected.