Rho sequences from fungi (Saccharomyces cerevisiae - Sc, Yarrowia lipolytica - Yl), entamoeba (Entamoeba histolytica - Eh), mycetozoans (Dictyostelium discoideum - Dd), alveolates (Tetrahymena thermophila - Tt), stramenopiles (Phytophthora ramorum - Pr) and plants (Arabidopsis thaliana - At) were aligned using ClustalX. Hydra magnipapillata (Hm) sequences were included as metazoan Rho sequences and Rab sequences as an external group. Only bootstrap values >700 are indicated on the NJ tree.