Figure 1.

CD44 immunohistochemical analysis of pancreatic islets. Sections of pancreas derived from diabetic recipient NOD mice (A, B, and C) or normal BALB/c mice (D) were incubated with KM81 anti-CD44 mAb (A, B, and D) or with the same concentration (5 μg/ml) of isotype-matched control Ig (IgG2b) (C). Bound antibodies were identified with biotinylated rabbit anti-rat IgG (7.5 μg/ml), using the avidin-biotin horseradish peroxidase detection system. CD44 is present on infiltrating MNCs and local cells of diabetogenic islets (A and B) but not on normal islet cells of BALB/c mice (D). CD44 staining of diabetogenic islets is not observed after their preincubation with isotype-matched control Ig (C). (A, ×176; B–D, ×352.)