Figure 2.

HA histochemical analysis of pancreatic islets. Sections from diabetic recipients (A and B) or normal BALB/c mice (C), pretreated [100 relative turbidity reducing units (rTRU)/ml] (B) or not pretreated (A and C) with hyaluronidase, were incubated with bHABP (2.5 μg/ml). Bound HA was identified by using the avidin-biotin peroxidase detection system. HA was detected in the connective tissue surrounding the islets (A) whereas normal islets (C) were only marginally stained by the same concentration of bHABP. Preincubation with hyaluronidase prevented the bHABP staining of the diabetogenic islets (B). (×352.)