Figure 2.

Staining of T cell hybridomas with the CII-DR4 and gp39-DR4 tetramers. Two CII-specific hybridomas are shown in A and B. Control T cell hybridomas in C and D were transfected to express TCRs of RA synovial clonal expansions and do not recognize CII (258–272) or HCgp39(263–275). All hybridomas were incubated with the indicated peptide–DR4 complexes at 20 μg/ml or 40 μg/ml with or without the anti-TCR mAb, H597. In separate wells, TCR expression was determined with biotinylated H597 followed by PE-avidin.