Figure 3.

Staining of RA synovial fluid cells with peptide–DR4 tetramers. Representative flow cytometric analyses are shown for synovial fluid cells from patients RA-6 (*0401/*1401) and RA-10 (*0101/*1601) stained with CII-DR4 (Top), gp39-DR4 (Middle), or anti-TCR Vβ17 (Bottom). For each histogram, CD14⁺ cells have been excluded, and the results are shown separately for the CD4⁺ and CD4⁻ populations. Results are also shown for separate samples in which anti-CD3 was coincubated with tetramer. Approximately 50 × 10³ cells were analyzed for each reagent combination. The gates used to determine positive cells are shown, and the percentages of cells staining with tetramer or anti-Vβ17 mAb are indicated in parentheses.