Figure 1.

Leishmania infection inhibits IL-12p40 transcription in murine macrophage cell line. RAW264.7 cells stably transfected with human IL-12p40-LUC construct were infected with L. major strain V1 and subsequently treated with 3 μg/ml CD40L ± 100 U/ml IFN-γ or 1 μg/ml LPS ± 100 U/ml IFN-γ. Samples were run in triplicate and mean relative luciferase units ± standard deviation is reported. * Infected values were significantly different from uninfected, stimulated controls (P < 0.05). † Uninfected, stimulated values were different from uninfected, untreated controls (P < 0.05). One representative of 3 independent experiments is presented.