Skip to main content
. Author manuscript; available in PMC: 2009 Apr 6.
Published in final edited form as: J Parasitol. 2008 Feb;94(1):84–93. doi: 10.1645/GE-1153.1

Figure 4.

Figure 4

Inhibition of IL-12p40Luc by Leishmania spp. products. RAW264p7 cells stably transfected with human IL-12p40-LUC construct were infected with L. major strain V1 or treated with parasite products for 16 hr followed by 8 hr of LPS (1 μg/ml) stimulation. (A) Parasite products (Live = live metacyclic infection; Lysate = freeze–thaw lysates; HK = heat-killed metacyclics). (B) Secreted products (V1 = live metacyclic infection; Neat, Dil, Conc = cultured parasite supernantants either diluted or concentrated). Samples were run in triplicate and mean relative luciferase units ± standard deviation is reported. * Infected values were significantly different from uninfected, stimulated controls (P < 0.05). † Untreated, stimulated values were different from untreated, media controls (P < 0.05). In each case 1 of 3 independent experiments is shown.