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. Author manuscript; available in PMC: 2009 Apr 6.
Published in final edited form as: J Parasitol. 2008 Feb;94(1):84–93. doi: 10.1645/GE-1153.1

Figure 6.

Figure 6

Inhibition of IL-12p40Luc by Leishmania does not involve autocrine MP factors; 1 × 106 RAW264.7 cells stably transfected with human IL-12p40-LUC construct were plated in lower wells and 1 × 106 were plated in upper wells of a transwell system. Cells were infected with L. major at a 5:1 ratio for 2 hr followed by IFN-γ (100 U/ml) stimulation for 16 hr and LPS (1 μg/ml) stimulation for 8 hr. Luciferase values were detected from cells in lower chambers. Samples were run in triplicate and mean relative luciferase units ± standard deviation is reported. * Infected values were significantly different from uninfected, stimulated controls (P < 0.05). † Uninfected, stimulated values were different from uninfected, untreated controls (P < 0.05). One representative of 3 independent experiments is shown.