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. Author manuscript; available in PMC: 2009 Apr 6.
Published in final edited form as: J Parasitol. 2008 Feb;94(1):84–93. doi: 10.1645/GE-1153.1

Figure 7.

Figure 7

Inhibition of IL-12p40Luc by Leishmania requires phagocytosis. RAW264.7 cells stably transfected with human IL-12p40-LUC construct were untreated (A, C) or pretreated with 1 μM cytochalasin D (B, D), then infected with L. major strain V1 and subsequently treated with 1 μg/ml LPS ± 100 U/ml IFN-γ. Samples were run in triplicate, treated with IFN-γ + LPS, and assessed for luciferase production (A, B) or endogenous levels of IL-12p40 by quantitative RT-PCR (C, D). Mean percentage expression ± standard deviation of is reported. * Infected values were significantly different from uninfected, stimulated controls (P < 0.05). One representative of 2 independent experiments is presented.