Table 1. Sequence of mutagenesis primers.

Mutation site	Sequence of primer
K69Q	5-GAA AGC TTG ACA GAT CCC AGT CAA TTA GAC TCT GGG A
K69R	5-GAA AGC TTG ACA GAT CCC AGT AGA TTA GAC TCT GGG A
K100Q	5-GAT ACT GGA ATT GGA ATG ACC CAG GCT GAC TTG ATC
K100R	5-GAT ACT GGA ATT GGA ATG ACC AGG GCT GAC TTG ATC
K292Q	5-TCG ATC AAG AAG AGC TCA ACC AAA CAA AGC CCA TCT G
K292R	5-TCG ATC AAG AAG AGC TCA ACA GAA CAA AGC CCA TCT G
K327Q	5-TGG GAA GAT CAC TTG GCA GTG CAG CAT TTT TCA GTT G
K327R	5-TGG GAA GAT CAC TTG GCA GTG AGG CAT TTT TCA GTT G
K478Q	5-GTG ATG AGA TGG TTT CTC TCC AGG ACT ACT GCA CCA G
K478R	5-GTG ATG AGA TGG TTT CTC TCA GGG ACT ACT GCA CCA G
K546Q	5-GAA GAC TTT AGT GTC AGT CAC CCA AGA AGG CCT GGA ACT
K546R	5-GAA GAC TTT AGT GTC AGT CAC CAG AGA AGG CCT GGA ACT
K558	5-TCC AGA GGA TGA AGA AGA GCA AAA GAA GCA GGA AG
K558R	5-TCC AGA GGA TGA AGA AGA GAG AAA GAA GCA GGA AG