FIG. 10. LRAT activity.

Two nmol of retinols were incubated with RPE microsomes and with homogenized HEKKLRAT cells for 10 min. The production of esters was monitored by HPLC measuring absorbance at 325 nm for all-trans-retinol (black bars) and 290 nm for all-trans-14,13-dihydroretinol (gray bars). Protein concentrations were not equalized. No activity was observed in controls with protein boiled for 10 min at 95 °C. Experiments were performed in triplicate.