Skip to main content
. Author manuscript; available in PMC: 2009 Apr 6.
Published in final edited form as: J Biol Chem. 2004 Sep 9;279(48):50230–50242. doi: 10.1074/jbc.M409130200

FIG. 6. RetSat activity toward all-trans-retinal.

FIG. 6

A, analysis of retinal conversion in RetSat-expressing cells. Tet-induced HEKK-Ret-Sat or untransfected cells were incubated overnight with pure all-trans-retinal (>99% pure by HPLC, assayed before incubation). Following incubation, retinals were derivatized with hydroxylamine, extracted, and analyzed by normal phase HPLC. The appearance of syn- and anti-oximes of 13,14-dihydroretinal was monitored at 290 nm (expected 6–8 min after injection, as indicated). The peak numbers represent 13-cis-retinol (1), all-trans-13,14-dihydroretinol (2), and all-trans-retinol (3). B, synthetic standards of 13,14-dihydroretinal derivatized with hydroxylamine were examined by normal phase HPLC in order to establish product elution profile. The inset shows the spectra of the different isomers of 13,14-dihydroretinal-oximes.