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. Author manuscript; available in PMC: 2009 Apr 6.
Published in final edited form as: J Biol Chem. 2004 Sep 9;279(48):50230–50242. doi: 10.1074/jbc.M409130200

FIG. 9. Identification of all-trans-13,14-dihydroretinol in various tissues.

FIG. 9

Retinoids were extracted from mouse liver (0.3 g, top left panel), kidney (0.2 g, top right panel), bovine retina (0.2 g, bottom left panel), and RPE (0.2 g, bottom right panel) and examined by normal phase HPLC. The elution of 13,14-dihydroretinol was monitored at 290 nm. Based on its retention time and absorbance spectrum, a peak corresponding to all-trans-13,14-dihydroretinol was identified in all tissues examined; it elutes on normal phase HPLC between 13-cis-retinol (1) and 9,13-di-cis-retinol (2). Other peaks corresponding to all-trans-retinol (3) and 11-cis-retinol (4; bovine retina and RPE) were also identified. The experiment was performed in duplicate from tissues of different animals. The yield of all-trans-13,14-dihydroretinol was slightly higher (<10%) by saponification of the extract before HPLC analysis. mAU, milliabsorbance units.