Figure 4.

High expression of the Cav-1^−/− MSF gene signature is associated with poor clinical outcome in breast cancer patients treated with tamoxifen monotherapy. A: Gene expression data from 60 ER-positive human breast tumors that were both micro- and macrodissected were analyzed for the expression pattern of proliferative genes up-regulated in Cav-1^−/− MSFs. A core of proliferation associated genes that are regulated by the RB/E2F pathway strongly co-segregated in this analysis. B: A Kaplan-Meier survival analysis was conducted, wherein the recurrence of those tumors in the highest one-third of overall expression was compared against the remainder of the cohort (*P < 0.0002). Patients in the high Cav-1^−/− MSFs gene expression group had a poor prognosis on tamoxifen monotherapy, with greater than a ∼2.6-fold reduction in disease-free survival.