Figure 5.

Status of the cytoskeleton in Cav-1^−/− MSFs. Mammary fibroblasts were isolated from the mammary glands of wild-type and Cav-1^−/− mice. Cells were then fixed and immunostained with antibodies against Cav-1 (N-20, Santa Cruz). In parallel, cells were directly labeled with fluorescein isothiocyanate-conjugated phalloidin, to stain actin filaments. Note that Cav-1^−/− MSFs exhibit thicker actin filaments. As expected, Cav-1^+/+ wild-type MSFs display a strong Cav-1 membrane staining. However, Cav-1 is absent in Cav-1^−/− cells. WT, wild-type; KO, Cav-1^−/−.