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Cav-1−/− MSFs show increased levels of TGF-β/Smad-responsive genes. Relative quantification of samples was assessed by arbitrarily setting the control cDNA value at 100, and changes in transcript levels of a sample were expressed as a multiple thereof (relative expression). The differences in the number of mRNA copies in each PCR reaction was corrected for using mouse 18S rRNA endogenous control transcript levels. (P < 0.05). WT, wild-type; KO, Cav-1−/−.
