Figure 8.

Cav-1^−/− MSFs show increased collagen I staining. Primary mammary fibroblasts were isolated from the mammary glands of wild-type and Cav-1^−/− mice. At passage 3, cells were allowed to reach confluency and were treated with ascorbic acid (40 μg/ml) for 24 hours. Ascorbic acid treatment facilitates collagen secretion. Then, cells were fixed and immunostained with rabbit polyclonal antibodies against collagen I (Novus Biologicals, CO). Note that Cav-1^−/− MSFs exhibit a higher collagen I content as compared with wild-type cells. Both panels are the same exposure time. WT, wild-type; KO, Cav-1^−/−.