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Increased numbers of NECs in lungs of C57BL/6 NEP−/− mice compared with NEP+/+ mice. Lung NECs were localized using rabbit anti-rat calcitonin gene-related peptide antibody. Positive signal (alkaline phosphatase, red). A: Representative positive NEC along an airway near a vessel in NEP−/− mouse lung. Large arrow points to stained NEC. B: NEP−/− mice have more NECs per length of airway than NEP+/+ mice (n = 4; *P < 0.05).
