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Increased α-SMA expression in distal vessels (10 to 50 μm) of NEP−/− mice compared with NEP+/+ mice in response to chronic hypoxia. Representative α-SMA (alkaline phosphatase, red) staining shown in mouse lung sections. A: NEP+/+ Nx. B: NEP+/+ 5 weeks Hx. C: NEP−/− Nx. D: NEP−/− 5 weeks Hx. Magnification = original ×200.
