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H&E-stained spleens show development of severe pathological lesions affecting primarily red pulp by 48 hours after infection. Spleens were harvested and fixed in 10% formalin after 1 hour (A and B), 24 hours (C and D), or 48 hours (E and F) after infection by Y. pestis CO92. Original magnifications: ×100 (A, C, E); ×400 (B, D, F).
