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. 2009 Mar;174(3):957–969. doi: 10.2353/ajpath.2009.080480

Figure 2.

Figure 2

Increased γ-cleavage of C99 in the presence of E693Δ mutation. HEK293 cells were transfected with C99WT and C99E693Δ constructs and cultured for 3 days. A: Cell lysates were subjected to Western blotting with C40. For molecular size markers, samples prepared from cells transfected with C59 and C50 constructs were also loaded on the gels. C83, equivalent to CTFα, was probably generated from transfected C99 and/or endogenous APP. Open arrowhead indicates an unidentified fragment of C99. B: Signals for C99 and C50 were quantified using a LAS-3000, and C50/C99 ratios were calculated. The columns and bars represent the means ± SD for three transfectants. *P = 0.0111 versus wild-type (WT) by unpaired Student’s t-test. The E693Δ mutation increased γ-cleavage of C99. Taken together with the results in Figure 1, this mutation was shown to increase Aβ production.