Figure 5.

Increased accumulation of the mutant Aβ in the endocytic pathway. COS-7 cells were transfected with APP_WT and APP_E693Δ constructs and cultured for 2 days. The cells were fixed, permeabilized, and blocked as described in Figure 4, and then stained with 11C (red) in combination with an anti-EEA1 antibody for early endosomes (green) (A), anti-M6PR antibody for late endosomes (green) (B), or anti-LAMP2 antibody for lysosomes (green) (C). The mutant Aβ was shown to accumulate in early and late endosomes and lysosomes more abundantly than wild-type (WT) Aβ.