Figure 3.

U0126 and LY294002 inhibit in vitro closure of a wound and cell growth of DU145 cells. A: For the in vitro wound-healing motility assay, DU145 cells were incubated in SFM, SFM with purified proepithelin (240 nmol/L) supplemented or not with 10 μmol/L U0126 or 30 μmol/L LY294002, or the combination of the two. Cells were analyzed as described in the legend for Figure 1. The experiment shown is representative of three independent experiments. B: Cell proliferation experiments were performed as described in the legend for Figure 1 in the presence of the same concentration of inhibitors. Data are the average of three independent experiments run in duplicates ±SD. *P < 0.05 compared with proepithelin-stimulated cells (second column).