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. 2009 Mar;174(3):1048–1064. doi: 10.2353/ajpath.2009.080676

Figure 2.

Figure 2

Localization of TAMs in PyMT Tumors. A: Immunohistochemistry was performed on serial frozen sections of late stage PyMT tumors. Negative irrelevant control antibody (a). F4/80 staining illustrates localization of macrophages in the stroma and diffusely in the tumor islands (b). Gr-1 staining is more sparse and restricted to stroma/peri-vascular areas of the tumor (c). Ly-6G staining is also restricted to stroma/peri-vascular areas of the tumor and appears to overlap with Gr-1 staining as indicated by black arrows (d). Scale bar = 50 μm. B: Localization of dextran labeling (red) cells within MacGreen tumor. C: Localization of CSF-1R (CD115) cells within MacGreen tumor. D: Localization of F4/80 cells within MacGreen tumor. E: Localization of Gr1 cells within MacGreen tumor. Inset ×2 magnification of DAPI-stained nuclei in merged image. B left panels green (EGFP) channel, middle panels red channel, right panels merged image with DAPI. Arrows indicate cells that are EGFP+, but do not express cell-type specified markers. Scale bars = 10 μm.