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. 2009 Mar;174(3):1097–1108. doi: 10.2353/ajpath.2009.080431

Figure 2.

Figure 2

A: Reciprocal oxidative stress and iron content in CD163high (HA-mac) and CD163low (foam cell) macrophages Left column: photomicrographs of macrophages in area identified as hemorrhage-associated (HA). Right column, photomicrographs of macrophages in area identified as foam cell rich (FC). Scale bars = 40 μm. Rows, immunolabeling as indicated, respectively for 8-oxo-guanosine (8-oxo-G); heme-oxygenase-1 (HO-1), and myeloperoxidase (MPO). The bottom row are double-labeled for CD163 (red)/iron (Perl’s stain; blue). Note Perl’s negative golden particles in the FC region are well described as OxLDL-derived ceroid.33 Brown is immunoperoxidase-DAB, and blue is hematoxylin counterstain. B–E: Ruptured plaque CD163+ macrophages, hemorrhage, iron, and IL10. Images correspond to area HA of (A), in serial sections, and are representative of the eight culprit plaques. Scale bars = 100 μm. B: − CD163 (blue immuno-alkaline phosphatase)/glycophorin C (erythrocytes, red immunoperoxidase) C: CD163/CD34. CD163 (red immunoperoxidase)/CD34 (endothelial cells, blue immuno-alkaline phosphatase); D: CD163/Perl’s iron histochemistry. CD163 (red immunoperoxidase)/iron (blue, histochemistry with Perl’s stain; ie, Prussian blue ferricyanide reaction). E: CD163/IL10 CD163 (blue immuno-alkaline phosphatase)/IL10 (red immunoperoxidase).