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. Author manuscript; available in PMC: 2009 Dec 15.
Published in final edited form as: J Immunol. 2008 Dec 15;181(12):8460–8477. doi: 10.4049/jimmunol.181.12.8460

FIGURE 6.

FIGURE 6

siRNA-mediated knockdown of FOXO3a reduces HIV-1-induced apoptosis. A, Up-regulation of FOXO3a mRNA in human PBMC infected with wild-type HIV-1-Bal 7 days after infection and in HeLa, but not in H9, cells infected with VSV-G-pseudotyped HIVΔ2GFP and HIVΔ3GFP (2 days postinfection) monitored by real-time PCR. Results are normalized to GAPDH and are expressed as fold induction relative to mock or eGFP control. B, mRNA knockdown of FOXO3a and selected target genes of FOXO3a (Bcl6, GADD45a, PTEN, and TRAIL) by real-time PCR in HeLa cells transfected with nonspecific (ns) siRNA or siRNA targeting FOXO3a. Results are normalized to GAPDH and are expressed as fold reduction relative to cells treated with control nonspecific siRNA. Western blot analysis of cell lysates obtained from infected and noninfected cells treated with FOXO3a RNAi and nsRNAi (C), and level of apoptosis (D) in the same cells 48 h following siRNA transduction are shown. The data are expressed as the means ± SE of three independent experiments. #, p < 0.05 with regard to reduction in apoptosis in HIV-1-infected cells treated with siRNA against FOXO3a relative to HIV-1-infected cells treated with nonspecific siRNA.