Table 1.

PCR Platforms, Extraction Methods, and Calibrators Used by Participating Laboratories

Lab#	Instrument / platform	Extraction method	Calibrator
1	LightCycler	QIAamp ultraSens	TIB plasmid*
2	LightCycler	QIAamp DNA mini kit	TIB plasmid
3	LightCycler	QIAamp minElute	TIB plasmid
4	LightCycler	Qiagen blood mini Kit	TIB plasmid
5	LightCycler	QIAamp DNA mini kit	TIB plasmid
6	LightCycler	Gentra pureGene	CMVAD169 quantitated viral DNA†
7	LightCycler	Roche highPure	TIB plasmid
8	LightCycler	Roche magnaPure	TIB plasmid
9	LightCycler	Roche magnaPure	In-house plasmid
10	LightCycler	Roche magnaPure	In-house culture
11	LightCycler	Roche magnaPure	In-house plasmid
12	LightCycler	Roche highPure	CMVAD169 quantitated viral DNA
13	ABI 7000	bioMerieux nucliSens	CMVAD169 quantitated viral DNA
14	ABI 7500	Qiagen M48 automated	In-house plasmid
15	ABI 7700	QIAamp DNA mini kit	CMVAD169 quantitated viral DNA
16	ABI 7700	Qiagen biorobot	In-house plasmid
17	ABI 7900HT	Gentra capture column	ATCC CMVAD-169
18	ABI Prism 7900HT	Roche magnaPure	In-house purified DNA
19	SmartCycler	Qiagen blood mini kit	CMVAD169 quantitated viral DNA
20	SmartCycler	QIAamp minElute	OptiQuant CMV viral DNA‡
21	iCycler	Qiagen viral kit	CMVAD169 quantitated viral DNA
22	iCycler	Qiagen blood mini kit	CMVAD169 quantitated viral DNA
23	COBAS AmplicorCMVmonitor	Amplicor CMV monitor	Kit quantitation standard§

^*TIB MolBiol.

^†Advanced Biotechnologies Inc.

^‡AcroMetrix.

^§Roche Diagnostics.