Figure 1.

Sensitivity of HRM assays for MGMT (A) and APC (B) methylation. Assays were run at annealing temperatures ranging from 64°C to 58°C using a touchdown protocol. Data were analyzed using software modules “Gene Scanning” and “Difference Plot.” Methylation standards are displayed as triplicates. Standards 100% blue lines, 50% red lines, 25% green lines, 10% pink lines, 1% yellow lines, 0.1% brown lines, and 0% orange lines.