Table 2.

Genes and Information of RT-PCR Primers and TaqMan Probes in the mERPR+HER2 Assay for the 7500 System

Gene ID	Gene symbol	Forward primer sequence	Reverse primer sequence	Reporter	Probe sequence∥
2099	ESR1*	5′-TCTGCAGGGAGAGGAGTTT-3′	5′-GGTCCTTCTCTTCCAGAGACTT-3′	FAM	5′-TGTGCCTCAAATCTA-3′
5241	PGR*	5′-TCGAGTCATTACCTCAGAAGAT-3′	5′-CCCACAGGTAAGGACACCATA-3′	TRE§	5′-TGACAGCCTGATGCTTCAT-3′
2064	ERBB2†	5′-CAGCCCTGGTCACCTACAA-3′	5′-GGGACAGGCAGTCACACA-3′	PHO§	5′-TGAGTCCATGCCCAATCC-3′
8021	NUP214‡	5′-CATTTGCTTTATAAAAGACCACTG-3′	5′-CCACTCCAAGTCTAGAACATCA-3′	VIC	5′-TCAGGAAATTCGGCGCCTT-3′
9360	PPIG‡	5′-GCCAACAGAGGGAAGGATA-3′	5′-GAGGAGTTGGTTTCGTTGTTA-3′	VIC	5′-ATGGTTCACAGTTCTTC-3′

^*ESR1 and PGR have at least four alternative splice variants. NM_000125, AF258449, AF258450, and AF258451 are the accession numbers of the variants for ESR1. NM_000926, AB085683, AB085844, and AB085845, are the accession numbers of the variants for PGR.

^†NM_004448 and NM_001005862 are the annotated accession numbers of the variants for ERBB2.

^‡NM_005085 and NM_004792 are the accession numbers of the variants for NUP214 and PPIG, respectively. For each of these genes, RT-PCR primers were designed to amplify a region shared by all listed splice variants. The amplicon sizes are 104-bp, 80-bp, 95-bp, 123-bp, and 61-bp, for ESR1, PGR, ERBB2, NUP214, and PPIG, respectively.

^§TRE and PHO labeled probes were provided by Applied Biosystems.

^∥All five TaqMan probes have minor-groove binder and non-fluorescent quencher at 3′ termini.