Table 3.
Classification of ER Status of the Discovery and Validation Sets Using the ΔΔCT Cutoff-Point and Clustering Methods
| Discovery (n = 67) |
Validation (n = 333) |
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| ΔΔCTcutoff-point |
Clustering |
ΔΔCTcutoff-point |
Clustering |
|||||||
| Allred TS* | IHC (% of total) | ER+ | ER− | ER+ | ER− | IHC (% of total) | ER+ | ER− | ER+ | ER− |
| 0 | 17 | 2 | 15 | 0 | 17 | 17 | 1 | 16 | 0 | 17 |
| 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 0 | 2 |
| ER−† | 17 (25%) | 2 | 15 | 0 | 17 | 19 (6%) | 1 | 18 | 0 | 19 |
| 3 | 2 | 0 | 2 | 0 | 2 | 4 | 0 | 4 | 0 | 4 |
| 4 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 1 | 1 |
| 5 | 3 | 3 | 0 | 3 | 0 | 6 | 6 | 0 | 4 | 2 |
| 6 | 2 | 2 | 0 | 2 | 0 | 31 | 31 | 0 | 28 | 3 |
| 7 | 12 | 12 | 0 | 12 | 0 | 110 | 110 | 0 | 110 | 0 |
| 8 | 31 | 31 | 0 | 31 | 0 | 161 | 161 | 0 | 160 | 1 |
| ER+‡ | 50 (75%) | 48 | 2 | 48 | 2 | 314 (94%) | 310 | 4 | 303 | 11 |
*
Allred total score.
†
Total number of specimens with Allred TS0 and TS2 in each set.
‡
Total number of specimens with Allred TS3 to TS8 in each set.