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. 2009 Mar;11(2):131–139. doi: 10.2353/jmoldx.2009.080129

Figure 5.

Figure 5

Characterization of variant c.1742A>G in exon 13 of the APC gene (patient 1172). A: Sequencing pattern of genomic DNA reveals the heterozygous substitution A>G. B: Agarose gel showing the RT-PCR product obtained with primers localized in exon 11F and in exon 15A-R in the patient (P) and a control (C). C: Diagram representing the normal and aberrant transcript. Arrows indicate primer positions. D: Sequencing pattern of the 501-bp and 618-bp fragments excised from the gel showing the deletion of exon 13 in the short fragment (above) and the (almost) complete lack of the mutant allele (arrow) in the full-length fragment.