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. 2009 Mar;11(2):131–139. doi: 10.2353/jmoldx.2009.080129

Figure 6.

Figure 6

Characterization of the duplication of exons 10 to 11 of the APC gene (patient 1199) detected by MLPA. A: Normalized peak areas showing the duplication of exons 10, 10a, and 11. B: Agarose gel showing the RT-PCR product obtained with primers localized in exon 9F and 13R in the patient (P) and a control (C). The faint bands above the main bands represent the products containing the alternatively spliced exon 10a (small arrows). C: Partial sequence showing the junction of exon 11 and exon 10 in the duplicated fragment (666 bp) excised from the agarose gel (without exon 10a). D: Diagram representing the order of the exons and the resulting premature stop codon in the duplicated exon 10. Arrows indicate primer positions.